Journal: Molecular Systems Biology
Article Title: Proteome-wide copy-number estimation from transcriptomics
doi: 10.1038/s44320-024-00064-3
Figure Lengend Snippet: Reagents and tools table
Article Snippet: CXADR-V5 was PCR amplified from pLX304 CXADR-V5 (Addgene #82723) with SpeI–MfeI restriction sites and cloned into SpeI–MfeI-digested pEN_TTmiRc2 (Addgene #25752) to yield pEN_TT CXADR-V5 (Addgene #220358). pEN_TT DNM1L-V5, luciferase-V5, and 3xFLAG-NUP37 (Addgene #192299) donor vectors were recombined into pSLIK neo (Addgene #25735) by LR recombination with LR Clonase II (ThermoFisher, 11791020) to obtain pSLIK DNM1L-V5 neo (Addgene #220363), pSLIK luciferase-V5 neo (Addgene #220364), and pSLIK 3xFLAG-NUP37 neo (Addgene #220361). pEN_TT CXADR-V5 donor vector was recombined into pSLIK hygro (Addgene #25737) by LR recombination to obtain pSLIK CXADR-V5 hygro (Addgene #220362). tet pLKO.1-shNUP37 v1 puro (Addgene #192343), tet pLKO.1-shLuciferase puro (Addgene #136587), pSLIK 3xFLAG-luciferase neo (Addgene #98392), pSuperior.retro.neo GFP shDNM1L (Addgene #220356), and pSuperior.retro.neo GFP shScramble (Addgene #220357) were obtained from authors of the source publications (Bajikar et al, ; Kashatus et al, ; Nagdas et al, ; Pereira et al, ; Wang et al, ).
Techniques: Recombinant, Luciferase, Sequencing, Cloning, Software, Bicinchoninic Acid Protein Assay